Kinesin Family Member C1 Promotes Proliferation of Colon Cancer Cells and its Correlation with Prognosis
LI Bing，CHEN Jing，YU Su-xiang
Abstract:
Objective To investigate the expression of KIFC1 in colon adenocarcinoma and its relationship with the clinicopathologic features and the prognosis of patients undergoing radical colectomy. Methods Immunohistochemical staining was used to analyze the expression of KIFC1 protein in 122 samples of colon adenocarcinoma and paracancer from January 2012 to December 2018 in the Baoding fourth central hospital, Tianjin tumor hospital and Baoding second hospital. According to the level of KIFC1 expression in colon adenocarcinoma samples, patients were divided into KIFC1 high expression group and KIFC1 low expression group. Clinical data related to the perioperative clinical features (age, gender, tumor T stage, differentiation level, clinical stage and lymph node metastasis), the correlation of KIFC1 expression and clinical features were analyzed, and the disease overall survival and progression-free survival of the two groups were analyzed by Kaplan-Meier method. The expression level of KIFC1 in colon cancer cell lines HCT116 and HT29 was knocked down by shRNA, and the effects of KIFC1 on the proliferation level of colon cancer cells lines were evaluated by MTT assay, colony formation assay, and detection of Ki67 and PCNA levels. Results Immunohistochemical results showed that among the 122 specimens of colon adenocarcinoma, 62 of them had low expression at protein level of KIFC1 and 60 samples showed high KIFC1 expression at protein level. High expression of KIFC1 level of KIFC1 and 60 samples showed high KIFC1 expression at protein level. High expression of KIFC1 was related with higher colon adenocarcinoma Tstage (P =0.040) and higher clinical stage (P =0.047).Patients with high expression of KIFC1 had poor overall survival and disease-free survival by using Kaplan-Meier analysis (P =0.0004 and P <0.0001 respectively). The colon cancer cell lines stably knocked down KIFC1 were established in vitro. MTT, cloning and proliferation-related protein assay showed that the cell proliferation rate decreased significantly (P <0.05), the number of colony formation decreased significantly(P <0.05), and the expression levels of proliferation-related protein Ki67 and PCNA decreased significantly(P <0.05). Conclusion KIFC1 protein is abnormally highly expressed in colorectal adenocarcinoma specimens, which is associated with high clinical staging of colorectal adenocarcinoma, leading to poor prognosis of patients. At the same time, KIFC1 has been proved to be able to regulate the proliferation of colon cancer cell lines, therefore, KIFC1 may become a new factor and therapeutic target for colon adenocarcinoma patients.
