Propofol Regulates the Effects of MiR-506 on the Proliferation, Migration, Invasion and Apoptosis of Osteosarcoma Cells
LIU Yuan-hao,ZHENG Yi-ming,WANG Bin
Department of Orthopedics, the Third People' s Hospital of Haikou, Hainan 571100, China
Abstract:
Objective To investigate whether propofol affects the proliferation, migration, invasion and apoptosis of osteosarcoma cells by regulating miR-506. Methods Osteosarcoma HOS cells were divided into NC group (untreated), Pro-L group (1 μg/mL propofol), Pro-M group (5 μg/mL propofol), Pro-H group (10 μg/mL propofol), miR-NC group (transfected with miR-NC), miR-506 group (transfected with miR-506 mimic), Pro+anti-miR-NC group (transfected with anti-miR-NC+10 μg /mL propofol) and Pro+anti-miR-506 group (transfected with miR-506 inhibitor+10 μg/mL propofol). Cell Counting Kit 8 (CCK-8) was used to measure cell proliferation. Western blotting was to evaluate CyclinD1, p21, MMP-2, MMP-9, Bcl-2, Bax protein expression and Transwell test to detect migration and invasion. Flow cytometry analyzed cell apoptosis, and fluorescence quantitative PCR was employed to determine the expression of miR-506. Results Compared with the NC group, the inhibition rate, p21, Bax protein level, apoptosis rate and miR-506 expression level of HOS cells in Pro-L, Pro-M and Pro-H groups gradually increased, while the protein level of CyclinD1, MMP2, MMP-9, Bcl-2, the number of migrating cells and the number of invasive cells gradually decreased, which showed a certain concentration-dependent, which the difference was statistically significant (P<0.05). The inhibition rate, apoptosis rate, p21 and Bax protein levels of HOS cells in the miR-506 group were higher than those in the miR-NC group, the number of migrating cells, the number of invading cells, CyclinD1, MMP-2, MMP-9 and Bcl-2 protein levels were lower than those of the miR-NC group, which the differences were statistically signi?cant (P<0.05). Compared with the Pro+antimiR-NC group, the Pro+anti-miR-506 group reduced the expression level, inhibition rate, apoptosis rate, p21 and Bax protein levels of miR-506 in HOS cells, and increased the number of migrating cells, the number of invasive cells, and the protein levels of CyclinD1, MMP-2, MMP-9 and Bcl-2, which the difference was statistically signi?cantly (P<0.05). Conclusions 1, 5, and 10 μg/mL propofol can up-regulate the expression of miR506, inhibit the proliferation, migration and invasion of osteosarcoma cells as well as promote their apoptosis.
