Effects of Salvianolic Acid B on Wound Healing and the Expression of Local bFGF and TGF-β1 in Diabetic Mice
LIU Qing-wu,ZHANG Jin-chao,CHEN Jia
Abstract:
Objective To explore the effect of salvianolic acid B (Sal B), the main component of Salvia miltiorrhiza, on wound healing of chronic wounds in diabetic mice and its molecular mechanism. Methods The experiment was divided into 4 groups, 30 db/db diabetic mice were randomly divided into model group, Sal B group and basic fibroblast growth factor (bFGF) group, 10 mice in each group. The control group consisted of 8 db/m mice. Wounds were prepared by full thickness skin resection on the back of mice in each group. The control group and the model group were externally used gelatin sponge containing normal saline. The bFGF group was externally used gelatin sponge containing bFGF and the Sal B group was externally used gelatin sponge containing Sal B. The external medication were wrapped with a disposable sterile dressing, the dressing were changed every other day and the wounds were taken to calculate the healing rate. The experimental period was 14 days. After the experiment, the wound skin was taken and stained with hematoxylineosin (HE) to observe the pathological changes. The expression of bFGF and transforming growth factor β1(TGF β1) genes and proteins were detected by real-time quantitative PCR and Western blotting protein quantitative methods respectively. Results On the 3rd, 7th, and 14th days after wound formation, the wound healing rate of the model group [(0.26±0.11)%, (0.43±0.12)%, (0.66±0.12)%] was lower than those of the control group [(0.63±0.09)%, (0.81±0.97)%, (0.98±0.02)%] (P<0.05). The wound healing rate of the bFGF group [(0.37±0.12)%, (0.59±0.07)%, (0.79±0.04)%] and Sal B group [(0.40±0.05)%, (0.58±0.05)%, (0.75±0.06)%] were higher than those of the model group (P<0.05). Compared with the control group [(0.95±0.16), (1.35±0.31)], the expression levels of bFGF mRNA and TGF-β1 mRNA in the model group [(0.19±0.05), (0.32±0.08)] were decreased (P<0.05). Compared with the model group, the expression of TGF-β1 mRNA in the bFGF group (1.31±0.33) was increased (P<0.01), and the expression of bFGF and TGF-β1 mRNA in the Sal B group [(0.71±0.25), (1.42±0.48)] were both increased (P<0.05). In addition, compared with the model group [(0.38±0.13), (0.26±0.12)], the expression of bFGF and TGF-β1 protein in the control group [(0.58±0.05), (0.55±0.16)], bFGF group [(0.64±0.12), (0.56±0.16)] and Sal B group [(0.63±0.10), (0.68±0.07)] were all increased (P<0.05). Conclusion Sal B can promote wound healing in diabetic mice. The mechanism may be to increase the level of wound growth factors and promote granulation tissue formation.
