The Effect of MicroRNA-133a-5p on the Migration and Invasion of Cholangiocarcinoma Cells Through Regulatingc-met
YANG Bo,LIU Xiao-fang
Department of Hepatobiliary-Pancreatic Surgery, Affiliated YantaiYuhuangding Hospital, Qingdao University, Yantai264000,China
Abstract:
Objective To investigate the expression of MicroRNA-133 (miR-133) in cholangiocarcinoma cells and to study the role of miR-133 gene expression in the migration and invasion of cholangiocarcinoma cells. Methods Real-time PCR (RT-PCR) was used to analyze the expression of miR-133 and its different matures(miR-133b、miR-133a-3p、miR-133a-5p) in cholangiocarcinoma cells QBC939. QBC939 cells were chosen and divided into three groups: natural growth (blank control group), cellgroups transfection with negative sequence (negative control group) and cell groups transfection with anti-miR-133a-5p(interference group). The expression of miR-133a-5pand c-met in the QBC939 cells of each group were measured by RT-PCR. The effect of miR-133a-5p on the migration and invasion of QBC939 cells was analyzed by Transwell assay. Results The expression of miR-133b(0.74±0.07), miR-133a-3p (0.99±0.12) and miR-133a-5p (1.00±0.06) in miR-133 was detected by RT-PCR,miR-133a-5p had the highest expression in cholangiocarcinoma cells QBC939(P<0.05). RT-PCR experiments confirmed that the expression of miR-133a-5p (0.70±0.08) in the interference group QBC939 cells was significantly lower than that in the negative control group (1.43±0.34, P<0.05) and the expression of c-met (0.09±0.02) in the interference group QBC939 cells was signi?cantly lower than that in the blank control group ( 1 . 01 ± 0.07, P<0.01). Transwell experiments confirmed that the migration (69.5 ± 8.3) and invasion (19.3 ± 3 . 3 ) abilities netof QBC939 cells in the interference group were signi?cantly lower than that of the negative and blank control groups (both P<0.01). Conclusion The low expression of microRNA-133a-5p can signi?cantly attenuate the expression of c-met and inhibit the migration and invasion of cholangiocarcinoma cells QBC939. The miR-133a-5pmaybecome a new target for the regulation of cholangiocarcinoma gene expression
