Observation of Shiguankang on Respiratory Chain Complex Ⅰ and Ⅳ Activities of Reflux Esophagitis
Model Rats HU Xi-bao,TIAN Jing-jing,YUAN Hong-xia
Department of Spleen and Stomach, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Abstract:
Objective To observe the effect of Shiguankang on the complex Ⅰ and Ⅳ activities of re?ux esophagitis (RE) model rats. Methods 64 male Wistar rats were randomly divided into 4 groups: normal control group，model control group，western medicine group and Shiguankang group, which 16 rats in each group. The model group was given the operation of esophagus through improved end-to-side anastomosis of the duodenum. Five days after the operation, the alive rats were randomly divided into model control group， western medicine group and Shiguankang group. From the sixth day after the operation, NS normal saline was given to the normal group and model control group, and Shiguankang was to the Shiguankang group, meanwhile, gavelans oprazole combined with mosapride were to the western medicine control group. Then the activities of complex Ⅰ and Ⅳ were observed. Results The activities of complex Ⅰ and Ⅳ of the model group were significantly lower than that in the normal group，which the difference was statistically significant (P<0.01). The activities of complex Ⅰ and Ⅳ of the western medicine group and Shiguankang group were higher than those in the model group，which the difference were statistically significant (P<0.05). Compared with the western medicine group，the activities of complex Ⅰ and Ⅳ of Shiguankang group increased unclearly，which the difference was not statistically significant (P>0.05). Conclusion By adjusting Qi movement of spleen and stomach，Shiguankang can increase the activities of complex Ⅰ and Ⅳ of esophageal tissue，maintain the integrity of Mitochondrial Respiratory Chain, increase the mitochondrial oxygen utilization ability and oxidative phosphorylation function. So it can improve the integrity of mitochondrial membrane structure and treat RE.
