Effect of Fufang Huangdai Tablets on Solt-Farber Primary Liver Cancer in Mice and Its Influence on Angiogenesis Effects
TI Zhen-yu,,GAO Xiao-peng,,QIAN Dong-wei
Second Department of General Surgery, Third Hospital of Xi' an, Xi' an (710018), China
Abstract:
Objective To observe the effects of Fufang Huangdai Tablets (FHT) on Solt-Farber primary liver cancer in mice and its in?uence on the angiogenesis effects. Methods Totally 50 Sprague-Dawley mice were divided into 5 groups, such as control group, model group, high dose group, medium dose group and low dose group. The models were made by Solt-Farber method in the groups except the model group. The mice in high dose group, middle dose group and low dose group were treated by FHT ( 280g, 140 and 70 mg/kg), the treatment continued for 18 weeks. After 12 h of the last treatment, the serum of mice was obtained and the livers were isolated. The levels of ALT, AST and TBIL were detected in the serum and the pathological and histological were also observed in the groups. The fluorescent-immunohistochemistry was performed in the groups to observe the expression of HIF- 1 α,VEGF and VEGFR- 2 . Results Compared with model group [ALT, ( 163 . 74 ± 16 . 45 ) IU/L; AST,(331.24±31.35) IU/L; TBIL, (86.11±8.51) IU/L],the serum liver function indexes of the rats in eachgroup were signi?cantly reduced, and the differences were statistically signi?cant (P < 0. 05). The indicators of each group were high dose group [ALT, (71.43±7.83) IU/L; AST, (112.52±12.58) IU/L; TBIL, (34.23±3.23)IU/L]; medium dose group [ALT, (105.32±10.13) IU/L; AST, (223.52±36.58) IU/L; TBIL, (42.93±4.02)IU/L], and low dose group [ALT, (122.34±12.11) IU/L; AST, (254.21±25.14) IU/L; TBIL, (51.27±5.26)IU/L]. Compared with the model group, the liver pathological histology showed that the canceration degrees were significantly promoted. Compared with model group (HIF- 1 α, 14 . 23 ± 1 . 28 ; VEGF, 11 . 52 ± 1.10; VEGFR-2, 16.34±1.65); the positive expression of HIF-1α, VEGF and VEGFR-2 in liver tissues of rats in each group were signi?cantly reduced and the relative expression was signi?cantly decreased, with statistically significant differences (P < 0 . 05). The indicators of each group were high dose group (HIF-1α, 3 . 92± 0.29; VEGF, 2.87±0.26; VEGFR-2, 3.45±0.40), medium dose group (HIF-1α, 6.36±0.65; VEGF, 5.34±0.51;VEGFR- 2 , 5 . 87 ± 0 . 60 ) and low dose groups (HIF- 1 α, 8 . 53 ± 0 . 81 ; VEGF, 7 . 76 ± 0 . 74 ; VEGFR- 2 ,8.12 ± 0 . 87 ). Conclusion FHT on Solt-Farber method induced primary liver cancer in mice has a good protective effect, and could inhibit the expression of HIF-1α, VEGF and VEGFR-2.
