LncRNA MALAT1 regulates the proliferation, apoptosis, migration of hepatoma cells through the miR-181a-5p/VCAM1 axis
JIA Guo-bing,GAO Jun-lin,LI Lian-sheng
Department of Hepatobiliary and Pancreatic Surgery, Qinghai Red Cross Hospital, Xining810000, China
Abstract:
Objective To investigate the impact of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on the proliferation, apoptosis, and migration of hepatoma cells by regulating miR-181a-5p/vascular endothelial cell adhesion molecule-1 (VCAM1) axis. Methods qRT-PCR was performed to measure MALAT1 and miR-181a-5p expression in liver cancer tissue, adjacent tissue and HL-7702, HepG2, Huh7, SK-HEP-1. The Huh7 cells were separated into five groups: ctrl group, si-NC group, si-MALAT1 group, si-MALAT1+anti-NC group, and si-MALAT1+anti-miR-181a-5p group; the MALAT1 and miR-181a-5p expression in cells of each group was measured by qRT-PCR; the cell proliferation was measured by CCK-8 assay and clone formation experiment; the cell apoptosis were measured by flow cytometry; the cell migration were measured by scratch assay; the protein expression of VCAM1, proliferating cell nuclear antigen (PCNA), B cell lymphoma/leukemia-2 (Bcl-2), E-cadherin, Vimentin in cells were measured by Western blot; relationship between MALAT1 and miR-181a-5p, between miR-181a-5p and VCAM1 were verified. Results Compared with ctrl group and si-NC group, the MALAT1, cell viability, number of clone formation, scratch healing rate, VCAM1, PCNA, Bcl-2, Vimentin protein expression in si-MALAT1 group decreased, the miR-181a-5p expression, E-cadherin protein and apoptosis rate increased (P <0.05); Compared with the si-MALAT1 group and the si-MALAT1+anti-NC group, there was no significant difference in the expression of MALAT1 in the si-MALAT1+anti-miR-181a-5p group (P >0.05), the cell viability, number of clone formation, scratch healing rate, VCAM1, PCNA, Bcl-2, Vimentin protein expression increased, the miR-181a-5p expression, E-cadherin protein and apoptosis rate decreased (P <0.05); MALAT1 and miR-181a-5p, miR-181a-5p and VCAM1 exhibit a negative regulatory relationship, respectively. Conclusion MALAT1 silencing may decrease the proliferation, and migration of Huh7 cells through miR-181a-5p/VCAM1 axis and enhance apoptosis.
