Mechanism of Xuanbai Chengqi decoction intervention on HIF-1α and inflammation related pathways to alleviate lung ischemia-reperfusion injury in rats
HAN Wen-heng,WANG Shun-hua,QU Peng-fei
Abstract:
Objective To investigate the effects of Xuanbai Chengqi decoction on lung ischemia-reperfusion injury in rats and analyze the mechanism of HIF-1α and inflammatory pathway in this process. Methods Forty healthy male SD rats were randomly divided into sham operation group (Sham), lung ischemia-reperfusion injury group (LIRI), Xuanfei group, and Xuanbai Chengqi decoction group. The degree of edema was assessed by measuring the wet-to-dry weight (W/D)ratio of lung tissue; pathological observations were conducted using HE staining of lung specimens, and the degree of injury was scored; meanwhile, protein expression of HIF-1α in lung tissue was measured by Western blot, and the levels of serum TNF-α and IL-1β were detected by ELISA. Results Compared to the Sham group, the W/D ratio increased in the LIRI group, Xuanfei group, and Xuanbai Chengqi decoction group. However, compared to the LIRI group, the Xuanfei group and Xuanbai Chengqi decoction group showed decreased performance in W/D ratio, with the Xuanbai Chengqi decoction group exhibiting lower data, and the differences were statistically significant (P <0.05). Under HE staining microscopy, varying degrees of damage were observed in the lung tissues of the LIRI group, Xuanfei group, and Xuanbai Chengqi decoction group. Compared to the LIRI group, the lung injury scores in the Xuanfei group and Xuanbai Chengqi decoction group decreased, with the Xuanbai Chengqi decoction group showing more significant improvement in lung injury, and the differences were statistically significant (P <0.05). The levels of HIF-1α, TNF-α, and IL-1β increased in the LIRI group, Xuanfei group, and Xuanbai Chengqi decoction group. However, compared to the LIRI group, the levels in the Xuanfei group and Xuanbai Chengqi decoction group decreased, with the Xuanbai Chengqi decoction group showing more significant reductions, and the differences were statistically significant (P <0.05). Conclusion Xuanbai Chengqi decoction may alleviate lung ischemia-reperfusion injury in rats by regulating the protein expression of HIF-1α and inhibiting inflammatory factor levels.
