Studies on the mechanism of Buzhong Yiqi decoction in regulating mucin-bile acid-microbiota metabolism for preventing inflammatory-cancerous transformation
DONG Guo-qiang,LIU Wei-jun,ZHANG Xing-zhou
Abstract:
Objective To investigate the mechanism of Buzhong Yiqi decoction in preventing intestinal inflammation-cancer transformation by regulating the gut mucin-bile acid-microbiota metabolism. Methods A colitis-associated cancer transformation model was established to conduct animal experiments and targeted metabolite analyses. The "solvent-shift" method and a co-culture cell model were utilized to design supersaturation stability and cellular transport assays. PCR, ELISA, and HPLC were applied to detect relevant biomarkers. Results Compared with the normal group, the model group showed deteriorated general status, with significantly increased mortality, tumor incidence, inflammatory factors, and mucin expression, whereas Buzhong Yiqi decoction administration significantly improved these indicators (P <0.05). The metabolic profile was disturbed in the model group and partially restored by Buzhong Yiqi decoction. Four intersection differential metabolites and five metabolic pathways were identified. Key pathways included bile acid biosynthesis and vitamin K metabolism. Mucin significantly increased the TSS or AUC of cholesterol and primary bile acids in FaSSIF (P <0.05), and the FE across mucus-secreting cell monolayers was significantly enhanced (P <0.05). Conclusion Under inflammatory bowel disease conditions, inflammatory factor-induced MUC2 overexpression promotes cholesterol absorption and maintains primary bile acid supersaturation, thereby elevating their microbial conversion to secondary bile acids. This process may promote inflammation-cancer transformation by affecting microbiota function and short-chain fatty acid metabolism. Buzhong Yiqi decoction inhibits the intestinal mucin-bile acid-microbiota metabolism, preventing and treating intestinal inflammation-cancer transformation.
