Distribution and antibiotic resistance of pathogens isolated from blood culture samples in Tianjin Nankai Hospital from 2022 to 2024
FANG Jie,GAO Qiao-ying,CHEN Ming-hui
Clinical Laboratory Department, Tianjin Nankai Hospital, Tianjin 300100, China
Abstract:
Objective To investigate the distribution and drug resistance of pathogens isolated from blood culture from 2022 to 2024, and to provide basis for clinical drug use. Methods 40 251 blood specimens from 2022 to 2024 were cultivated with automated blood culture systems; bacteria identification and drug resistance were carried by Vitek MS and compact bacteria identification system, with susceptibility results interpreted according to CLSI standards. Results There were 40 251 blood specimens, with a contamination rate of 1.65%(665/40 251). 4205 strains of pathogens were isolated, including 2120 gram-positive bacteria, 1749 gram-negative bacteria, 174 fungi and 162 anaerobic bacteria. The pathogens were mainly distributed in Hepatobiliary and Pancreatic Surgery Department, ICU, Gastrointestinal Surgery Department, EICU, Medical oncology Department, Respiratory and Critical Care Department, and Infectious Diseases Department. Mainly gram positive cocci were coagulase negative staphylococcus and Enterococcus; gram negative bacteria were mainly Escherichia coli, Klebsiella pneumoniae, enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter baumannii, who were resistant to carbapenems. Gram positive cocci were sensitive to vancomycin, tigecycline and linezolid, and 1 Enterococcus faecium was resistant to vancomycin; gram negative bacteria exhibit varying degrees of resistance to carbapenem antibiotics. Conclusion There are many types of pathogens isolated and gram negative bacteria have severe resistance to common antibiotics, especially carbapenem-resistant Klebsiella pneumoniae. The distribution of pathogens and drug resistance should be strengthened to provide an optimal antimicrobial therapy.
