Effect of the fermented broth of Mieyou decoction on TLR4/MyD88/NF-κB signaling pathway in mice with Helicobacter pylori infection-related gastritis
ZHANG Xu-yuan,GUO Xuan
School of Integrated Chinese and Western Medicine, Hunan University of Traditional Chinese Medicine, Changsha41000, China
Abstract:
Objective To investigate the therapeutic effect and mechanism of the fermentation broth of Mieyou decoction on mice with Helicobacter pylori infusion-associated gastritis based on the Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear transcription factor-κB (NF-κB) signaling pathway. Methods Forty-four mice were marked as the blank group (10 mice) and the model group (34 mice). After 7 days of adaptive rearing, Helicobacter pylori bacterial liquid was gavaged once every other day for a total of 6 times. After 14 days of conventional feeding, 2 mice from each group were selected for the Rapid Urease Test (RUT) and Giemsa staining to detect whether the modeling was successful. The remaining mice were divided into the control group, the model group, the Mieyou decoction group, the fermentation broth group and the Western medicine group, with 8 mice in each group. The drug intervention lasted for 14 days. The mice were sacrificed to obtain gastric tissues, and RUT was used to detect the H.pylori eradication rate of the mice. Giemsa and HE staining were used to observe the colonization and inflammatory infiltration of H.pylori in mice. The protein and mRNA levels of TLR4, MyD88 and NF-κB in mouse gastric tissues were detected by IHC method and real-time fluorescence quantitative PCR. The expression changes of TNF-α and IL-8 in the serum of mice were evaluated by ELISA. Results Compared with the blank group, a large number of inflammatory cell infiltrations and the presence of Helicobacter pylori could be observed in the gastric mucosa of mice in the model group. The protein and mRNA levels of TLR4, Myd88 and NF-κB, as well as the serum contents of TNF-α and IL-8, were significantly increased (P <0.05). Compared with the model group, the gastric mucosal inflammation of mice in the Mieyou decoction group, the fermentation broth group and the Western medicine group improved, and the expression levels of each protein and mRNA as well as the serum contents of TNF-α and IL-8 all decreased (P <0.05). Compared with the Mieyou decoction group, the gastric mucosal inflammation of mice in the fermentation broth group and the Western medicine group improved, and the levels of expression of each protein and mRNA as well as the serum contents of TNF-α and IL-8 decreased (P <0.05). Conclusion Both the Mieyou decoction and its fermentation broth can inhibit the colonization of Helicobacter pylori, reduce gastric mucosal inflammation, and the fermentation broth has better therapeutic effects. Its therapeutic mechanism is related to the inhibition of the TLR4/MyD88/NF-κB signaling pathway.
