Study on the regulatory effects of resveratrol on trimethylamine N-oxide level and intestinal flora abundance in atherosclerosis model rats
WANG Yu-tao,YANG Li-ke,WANG Hua-yu
Abstract:
Objective To investigate the regulatory effects of resveratrol (RES) on trimethylamine N-oxide (TMAO) level and intestinal flora abundance in atherosclerosis (AS) rats. Methods Twenty-four rats were randomly divided into control group, AS group and RES intervention group. Rats in the AS group and RES intervention group were fed with abdominal aortic balloon injury method combined with high-fat chow for 2 weeks to replicate the AS model. The drug was administered by gavage starting on the next day of modeling, once daily for 4 weeks. Aortic hematoxylin-eosin (HE) staining and lipid levels were used to verify the effect of RES intervention in AS, and the abundance of intestinal flora in intestinal contents was analyzed using metagenomics. Results Compared with the control group, serum triglyceride (TG), total cholesterol (TC), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) and TMAO level of AS group rats were elevated. The intestinal bacterial biodiversity was reduced. At the level of the phylum, the relative abundance of Firmicutes and the Actinobacteria were down-regulated, and the relative abundance of Bacteroidetes and Uroviricota was up-regulated. At the class level, the relative abundance of Actinomycetia, Bacilli and Clostridia was down-regulated, the relative abundance of Bacteroidia, Erysipelotrichia and Gammaproteobacteria was up-regulated. At the genus level, the relative abundance of Bacteroides, Escherichia, Muribaculum, and Parabacteroides was up-regulated, and the relative abundance of Bifidobacterium, Lactobacillus, Ligilactobacillus, Limosilactobacillus, Methanosphaera and Romboutsia was down-regulated. In comparison to the AS group, the serum TC, TNF-α, IL-6 and TMAO of rats in RES intervention group were decreased. RES up-regulated the biodiversity of intestinal flora and the relative abundance of Firmicutes, Bacilli, Clostridia, Bifidobacterium, Lactobacillus, Ligilactobacillus, Limosilactobacillus, and Romboutsia in AS model rats, and down-regulated the relative abundance of Uroviricota, Bacteroidetes, Bacteroidia, Erysipelotrichia, Gammaproteobacteria and Muribaculum, with all differences were statistically significant (P <0.05). Conclusion RES can regulate lipid levels in AS rats, reduce the expression of TNF-α, IL-6, and TMAO, upregulate the abundance of beneficial microbiota, downregulate the abundance of harmful microbiota, and modulate the diversity of the gut microbiota.
