Metabolomic clinical analysis of sepsis combined with acute lung injury
XUE Hai-ling,WANG Rui,ZHANG Ai-min
Abstract:
Objectives Using non-targeted metabolomics methods to search for specific metabolite markers and metabolism pathways in sepsis with acute lung injury (ALI), providing new basis and evidence for clinical diagnosis and treatment. Methods Metabolomics analysis was performed on serum samples from three groups of subjects with sepsis accompanied by ALI (23 cases), without ALI (14 cases), and healthy individuals (15 cases) during the same period using ultra-high performance liquid chromatography/mass spectrometry(UPLC/MS). Differential metabolites and metabolism pathways were statistically analyzed. Results Compared with the sepsis without ALI group, the sepsis with ALI group showed an increase in the concentrations of inflammation related metabolites 7-oxocholesterol and hippuric acid, antioxidant metabolites 7-O-glucoside, kaempferol glucoside, and demethylangin, energy related metabolites PGK1, DL-3-hydroxyhexanoic acid, and fibrinogen B3, and a decrease in the concentrations of anti-inflammatory metabolites glycerodeoxycholic acid, antioxidant metabolite 2-hydroxyestrone, endogenous metabolite N-(3-chlorobenzoyl) glycine, and drug (M) - alkaloids. The metabolism pathways with significant changes include estradiol metabolism, ammonia recovery, histidine metabolism, and bile acid metabolism. Conclusion Non-targeted metabolomics methods were used to screen for inflammation related metabolites, antioxidant metabolites, energy related metabolites, and anti-inflammatory metabolites with significant changes in the serum of sepsis with ALI patients. At the same time, there were disturbances in estradiol metabolism, ammonia recovery, histidine metabolism, and bile acid metabolism pathways in this group of patients.
