Mechanism of Panax notoginseng saponins regulate Wnt/β-catenin signaling pathway on steroid induced femoral head necrosis in rats
CUI Ya-nan,SONG Shao-feng,DU Zhi-juan
The Second Department of Trauma, Zhangjiakou Second Hospital 075000，China
Abstract:
Objective To explore the effects of different doses of Panax notoginseng saponins on bone mechanical parameters and Wnt/β-catenin signal pathway molecules in rats with steroid induced femoral head necrosis. Methods 50 SD rats were divided into control group, model group, low dose notoginsenoside group, medium dose notoginsenoside group and high dose notoginsenoside group. The rat model of steroid induced femoral head necrosis was induced by 20 mg/(kg·d) methylprednisolone. It was injected 3 times a week for 3 weeks, and the control group was injected with the same amount of normal saline. After successful modeling, each treatment group was given 5, 10, 20 mg/(kg·d) doses of Panax notoginseng saponin by gavage for 6 weeks, and the control group and model group were given the same amount of normal saline by gavage. The bone mineral density, bone biomechanical indexes and bone morphological indexes were measured at the end of the experiment; Serum tartrate resistant acid-base phosphatase (TRAP), urinary type I collagen cross-linked N-terminal peptide (U-NTX), bone alkaline phosphatase (BALP), estradiol (E2), urinary type collagen cross-linked C-terminal peptide (U-CTX), osteocalcin (BGP) and other bone transformation indicators were detected by ELISA; The mRNA and protein expression levels of Wnt5a and c-Myc of Wnt/ β-catenin pathway molecules in bone tissue were detected by qPCR, Western blot and immunohistochemistry. Results Panax notoginseng saponins can significantly increase the bone mineral density of femur and spine in osteoporosis rats, and improve the level of biomechanical parameters of femur (P < 0.01); The percentage of cortical bone area, average trabecular thickness, trabecular area and trabecular volume were increased (P < 0.01), the levels of BALP and E2 in serum were increased, and the levels of BGP, TRAP, U-NTX and U-CTX in serum were decreased (P < 0.01); The mRNA and protein expression levels of Wnt5a/ β-catenin pathway molecules were significantly increased (P < 0.01). Conclusion Panax notoginseng saponins can improve the bone mechanical parameters of rats with estrogen induced femoral head necrosis and regulate the expression of Wnt5a and c-Myc, which is conducive to the treatment of estrogen induced femoral head necrosis.
