Effects and mechanism of Maiguan Fukang Tablets combined with Tangbi Waixi Prescription on diabetic angiopathy
SHEN Yong -ye,LI Ying,SONG Zi -teng
Abstract:
Objective To establish the hind limb ischemia model in diabetic rats and explore the effects of Maiguan Fukang tablets combined with Tangbi Waixi Prescription on vascular endothelial injury in diabetic rats and its role in promoting angiogenesis. Methods The rat diabetes model was established by high fat diet and intraperitoneal injection of streptozotocin. Based on the measurement of blood glucose levels, the successfully modeled rats were ligated with the left hind limb femoral artery to establish the lower limb ischemia model of diabetic rats. After modeling, the model rats were randomly divided into the model group, Tangbi Waixi Prescription combined with Maiguan Fukang tablets high, medium, and low dose group. After 21 days of administration, the rats were sacrificed and blood samples and left hind limb gastrocnemius muscle tissues were taken. The levels of TXA2, ET-1, NO, eNOS, VEGF, sVCAM-1, and CRP were detected by ELISA. HE staining was used to observe the morphology of muscle cells. The expression of the CD31 marked microvascular density was determined by immunohistochemical staining. Results After treatment with Maiguan Fukang Tablets combined with Tangbi Waixi Prescription, no obvious atrophy and necrosis were found in the gastrocnemius muscle tissue of rats by HE staining, and the vascularcompared with the model group, the changes of endothelial function tended to be normal. When relevant indicators were as follows. The TXA2 levels in the serum of rats in the high-, medium-, and low levels of ET-1 all decreased, with the highest dose group being the most significant ( <0.001). The NO levels in the high-dose group significantly increased ( <0.001). There was a significant increase in VEGF levels in the high-and medium-dose groups ( <0.01). The immunohistochemical results showed that the high-dose group showed a significant increase in number of microvasculature formation ( <0.01). Conclusion Maiguan Fukang Tablets combined with Tangbi Waixi Prescription can maintain vascular endothelial function by regulating TXA2, ET-1 and NO levels, and promote angiogenesis by increasing the expression of VEGF, thereby improving hind limb ischemia in diabetes rats.
