Study on synergistic mechanism of astragalus in Brevinin-2Ta for wound infection-resistant Acinetobacter baumannii
XU Yang,ZHOU Xin,,NIU Xin-yue
Abstract:
Objective To investigate the synergistic effect and mechanism of the traditional Chinese medicine Astragalus astragalus in the treatment of antibacterial peptide Brevinin-2Ta in the treatment of wound infection resistant Acinetobacter baumannii. Methods 60 SD rats were randomly divided into blank group, antibacterial peptide group and antimicrobial peptide + astragalus group, and the wound wound of rats was taken on the 3rd and 15th days after successful modeling, the wound of the wound was taken, the tissue at the wound was taken, the bacterial morphology was observed under electron microscopy, and the expression content of carO gene was determined by fluorescent PCR. Results The carO of the blank group on day 3 after administration was 28.770±8.829, antimicrobial peptide group was 14.721±3.879; antimicrobial peptide + astragalus group was 11.768±2.752. The expression of carO in the antimicrobial peptide group and the antimicrobial peptide + astragalus group was significantly inhibited compared with the blank group (P<0.05), while there was no significant difference in the expression of carO between the antimicrobial peptide group and the antimicrobial peptide + astragalus group (P>0.05). At the 15th day after administration, the carO of the blank group was 28.113±4.996; antimicrobial peptide group was 17.490±2.648; antimicrobial peptide + traditional Chinese medicine group was 11.082±2.476, and there were statistical differences between the blank group, antimicrobial peptide group and antimicrobial peptide + astragalus group (P<0.05). After treatment, electron microscopy showed that on the 3rd day, the bacterial cell membrane structure of the antibacterial peptide group was destroyed and adhered to each other, and a large number of bacterial necrotic adhesions could be seen in the antibacterial peptide + astragalus group; On the 15th day, a large amount of bacterial necrosis was seen in the antibacterial peptide group and the antibacterial peptide + astragalus group, and the antibacterial peptide + astragalus group was better than the antibacterial peptide group. Conclusion The traditional Chinese medicine Astragalus has a certain synergistic effect in the treatment of antibacterial peptide Brevinin-2Ta in the treatment of wound infection resistant Acinetobacter baumannii, and its mechanism may be related to the inhibition of carO expression.
