Study on the mechanism of Sijunzi plus Radix Cyathulae Decoction intervention on the P38MAPK/CyclinD1-CDK4 pathway in promoting the healing of the refractory wound in rats’ lower limbs
SHEN Liang,CHENG Wei,WANG Yun-fei
Surgery of Chinese Medicine, Longhua Hospital Affiliated to Shanghai University of TCM, Shanghai200032, China
Abstract:
Objective To explore the mechanism of how “Sijunzi plus Radix Cyathulae Decoction” interfere the P38MAPK/CyclinD1-CDK4 pathway in promoting the healing of the refractory wound in Rats’ lower limbs. Methods The models of full-layer skin defect and refractory wound on the right lower limb were established，and 64 male SD rats were randomly divided into Normal Saline group，“Sijunzi plus Radix Cyathulae Decoction” group，“Sijunzi Decoction” group and Radix Cyathulae group. Each group was given normal saline and corresponding traditional Chinese medicine intragastric treatment. Wound granulation tissue and new blood of rats in each group were respectively extracted on the 7th day and on the 14th day. The contents of cyclinD1 and CDK4 in granulation tissue were detected by immunohistochemistry and image analysis. The p38MAPK protein level in granulation tissue was detected by Western blot. Results On day 7 after modeling, the p38MAPK in“Sijunzi Decoction” group and “Sijunzi plus Radix Cyathulae Decoction” group were markedly higher than those in Normal Saline group, there was significant difference(P<0.01); there was no significant differences between “Radix Cyathulae” group and Normal Saline group (P>0.05); there was no significant difference between Sijunzi decoction group and Sijunzi Jiachuan Niushifang group (P>0.05). On day 14 after modeling, there was no significant differences of P38MAPK concentration among the four groups (P>0.05). On day 7 and 14 after modeling: there was no significant differences about expression of CyclinD1 and CDK4 among the four groups(P>0.05). Conclusion The prescription of “Sijunzi plus Radix Cyathulae Decoction” can regulate the P38MAPK/CyclinD1-CDK4 signal transduction pathway and promote the proliferation of fibroblasts, which may be the mechanism of promoting granulation tissue hyperplasia and wound healing.
