The protective effect of Qingyi decoction combined with bone marrow mesenchymal stem cells on the air-blood barrier in severe acute pancreatitis with lung injury
LIU Xiao-jun,BAI Bu-yue,WU Ping
Abstract:
Objective To explore the protective effect of Qingyi decoction combined with bone marrow mesenchymal stem cells (BMSCs) on the lung air-blood barrier in severe acute pancreatitis lung injury (SAPALI). Methods The SAPALI model was established by retrograde injection of 3.5% sodium taurocholate into the bile and pancreatic ducts of rats. Fifty male rats were evenly divided into sham operation group, model group, Qingyi decoction group, BMSCs group and Qingyi decoction combined with BMSCs group. HE staining detects pancreatic and lung tissue damage. ELISA was used to detect the expression levels of serum lipase, TNF -a and IL -6.Immunohistochemistry was used to detect changes in levels of lung oxidative stress -related indicators myeloperoxidase (MPO) and 4-hydroxynonenoic acid (4-HNE). Immunofluorescence analysis and Western Blotting were used to detect the expression levels of alveolar epithelial cells and microvascular endothelial cells. Results Qingyi decoction can significantly improve the pulmonary microenvironment caused by severe acute pancreatitis.Compared with the BMSCs group, combined use of BMSCs can down-regulate the expression of TNF-a and IL-6 in bronchoalveolar lavage fluid as well as the expression of MPO and 4-HNE in lung tissue. The above results suggest that Qingyi decoction can promote BMSCs to repair the lung air-blood barrier. Combined treatment with Qingyi decoction and BMSCs increased the expression of alveolar epithelial cells and pulmonary microvascular endothelial cells in SAPALI. Conclusion Qingyi decoction combined with BMSCs can promote the repair of lung air-blood barrier in SAPALI by reducing the level of inflammatory response and oxidative stress.
