Correlation between serum LncRNA MAGI2-AS3, miR-374b-5p expression levels and Gleason grading score in patients with prostate cancer
YANG Lin,LIANG Xiang,DENG Jin-chao
Abstract:
Objective To explore the correlation between the expression levels of serum LncRNA MAGI2-AS3 and miR-374b-5p and Gleason grading score in patients with prostate cancer. Methods Prostate cancer patients (n=110) who received treatment at our hospital from November 2021 to November 2023 were collected as the prostate cancer group, and benign prostate disease patients (n=110) who underwent physical examination were as the reference group. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was applied to detect the expression of LncRNA MAGI2-AS3 and miR-374b-5p in serum; Spearman method was applied to analyze the correlation between serum LncRNA MAGI2-AS3, miR-374b-5p and Gleason grading score; Pearson method was applied to analyze the relationship between serum LncRNA MAGI2 -AS3 and miR -374b -5p;multivariate Logistic regression was applied to analyze the factors influencing the occurrence of prostate cancer. Results Compared with the reference group, the serum LncRNA MAGI2-AS3 expression level in the prostate cancer group decreased (p<0.05), while the miR-374b-5p expression level increased (p<0.05). The serumLncRNA MAGI2-AS3 and miR-374b-5p expression levels in prostate cancer patients were negatively correlated(r=-0.673, <0.05). Multivariate Logistic regression analysis showed that LncRNA MAGI2-AS3 was a protective factor affecting the occurrence of prostate cancer (p<0.05),and miR -374b -5p was a risk factor affecting the occurrence of prostate cancer (p<0.05). Compared with the low -risk group, the serum LncRNA MAGI2 -AS3 expression level in the medium and high-risk groups decreased sequentially (p<0.05), and the miR-374b-5p expression level increased sequentially (p<0.05). The serum LncRNA MAGI2-AS3 in prostate cancer patients was negatively correlated with Gleason grading score (r=-0.497, p<0.05), and the expression level of miR-374b-5p was positively correlated with Gleason grading score (r=0.456, p<0.05). Conclusion The expression level of serum LncRNA MAGI2-AS3 in prostate cancer patients is greatly reduced, while the expression level of miR-374b-5p is greatly increased, which are related to Gleason grading score.
