The role of the theory of main body fluid in large intestine in aged constipation model rats
ZHANG Xiao-nan,LIN Ai-zhen,SUN Guang-jun
Wuhan No.1 Hospital, Wuhan(430022), China
Abstract:
Objective To explore the mechanism of action of the theory of main body fluid of large intestine in aged constipation model rats. Methods The rat model of senile constipation was established by oral administration of compound difenoate 10 mg/kg for 2 weeks. The rats were randomly divided into model group, Chinese Medicine Group and lactulose group with 10 rats in each group, and the blank control group with 10 rats in each group. After 2 weeks, the fecal water content and intestinal carbon powder propulsive rate were measured, and Caesalpinia sappan eosin staining was used for histological analysis The mRNA levels of AQP3, AQP4 and AQP8 in colon were detected by real-time quantitative PCR. Results Showed that compared with the normal group, the water content of feces and the propulsive rate of activated carbon in the model group were significantly decreased, the thickness of colonic muscle layer was decreased, the amount of mucin was decreased, and the intestinal barrier structure was damaged The expressions of AQP3, AQP4 and AQP8 mRNA were up-regulated. Compared with the model group, the fecal water content and activated carbon propulsive rate of rats treated with Yichangtong were significantly increased, the damaged intestinal barrier structure was obviously recovered, the thickness of colonic muscle layer and the amount of mucin were increased The expressions of AQP3, AQP4 and AQP8 mRNA were down-regulated. The water content of the feces and the expression of aquaporin in the Chinese herbal medicine group were better than those in the lactulose group. Conclusion The effect of Yichangtong on senile constipation model rats is clear, and its main mechanism can quickly recover the damage of intestinal pathomorphology, and increase the water content of feces by regulating the levels of AQP3, AQP4 and AQP8 mRNA in colonic mucosa, improve constipation.
