Influence of ZFP28 on proliferation, metabolism and motility of renal clear cell carcinoma cells
BIAN Jian-qiang,LI Chang-jian,ZHU Guang-bin
Department of Urology, Tianjin Hospital, Tianjin(300211), China
Abstract:
Objective To investigate the influence of ZFP28 on proliferation, metabolism, and motility of clear cell renal cell carcinoma (ccRCC) cells, along with its underlying mechanism. Methods Immunoblotting and qPCR were employed to assess the expression of ZFP28 in 786-O cells and HK-2 cells. Cell proliferation and metabolism capacity were determined using CCK-8 assay, colony formation, Edu, ATP synthesis assay, glucose uptake assay, and flow cytometry. The impact on the AKT/mTOR pathway was evaluated through immunoblotting, while Transwell assays were conducted to reveal effects on cell migration and invasion. Results ZFP28 was highly expressed in ccRCC cells compared with normal proximal renal tubule epithelial cell line HK-2 (P <0.05). Down-regulation of ZFP28 by siRNA inhibited the growth, colony formation, ATP synthesis and glucose uptake of 786-O cells, and inhibited the migration and invasion of 786-O cells (P <0.05). ZFP28 down-regulated the AKT/mTOR pathway of 786-O cells in ccRCC cells (P <0.05). Conclusion ZFP28 inhibits the growth, metabolism, and motility of clear cell renal cell carcinoma cells through the AKT/mTOR axis, suggesting its potential as a therapeutic target for ccRCC.
