Based on wnt/β-catenin signaling pathway to explore mechanism of Qingyi decoction in promoting repair of alveolar epithelial cell damage in severe acute pancreatitis
WANG Yin,BAI Bu-yue,CHEN Hai-long
Abstract:
Objective To explore the role of "Wnt/β-catenin" signaling pathway in the pathogenesis of lung injury in severe acute pancreatitis (SAP) and the intervention effect of Qingyi decoction (QYD). Methods 40 rats were divided into Sham group, sham operation group (Sham+QYD), SAP group, and QYD treatment group (SAP+QYD). The SAP model was established by retrograde injection of 3.5% sodium taurocholate through the biliopancreatic duct. After 48 hours of modeling, pancreatic and lung tissues were taken, and tissue pathological damage was detected by hematoxylin-eosin (HE) staining, the lung wet/dry weight ratio (W/D) was measured using vacuum drying method, serum IL-6 levels were detected using enzyme-linked immunosorbent assay (ELISA), and lung tissue apoptosis was detected using TUNEL assay and Western blot (WB). Immunofluorescence assay was used to detect double staining of CyclinD1 and SFTPC, β-catenin and SFTPC. WB method was used to detect the expression of key proteins in the "Wnt/β-catenin" signaling pathway and downstream cyclins. Results Compared with the Sham group, HE slices of SAP group rats showed severe pancreatic and pulmonary lesions, significantly increased serum IL-6 levels, and decreased SFTPC expression, indicating severe damage to alveolar epithelial cells. Compared with SAP rats, QYD treatment showed varying degrees of improvement in various indicators such as pancreatic and lung tissue pathological scores, W/D, IL-6 content, apoptotic proteins Bcl-2/Bax, and the expression of key proteins in the Wnt/ β-catenin signaling pathway and downstream cyclins was significantly upregulated. The immunofluorescence double staining results showed that the QYD treatment group had more double stained cells, and the above differences were statistically significant (P<0.05). Conclusion QYD through activate Wnt/β-catenin signaling pathway plays a crucial role in promoting the repair of acute pancreatitis related lung injury by reducing apoptosis of alveolar epithelial cells.
