The role of intestinal fatty acid binding protein and D-lactic acid in the early diagnosis of intestinal necrosis in incarcerated hernia
MAN Yi，SHI Song-li，LI Bao-shan
Abstract:
Objective To investigate the role of intestinal fatty acid binding protein (I-FABP) and D-lactate(D-LAC) in the diagnosis of intestinal necrosis in incarcerated hernia. Methods Sprague Dawley rats were used for incarcerated hernia model, and then divided into control group (n=18) and experimental group (n=18). At 30 min, 2 h, 4 h, 6 h, 8 h and 12 h after operation, the levels of D-LAC and I-FABP in peripheral blood of the two groups were detected by ELISA. RT-qPCR was used to identify the expression of I-FABP in the intestinal tissue of incarcerated hernia. Intestinal necrosis was determined by gross specimen, HE staining and Chiu's score. Results Compared with the control group, the experimental group showed typical intestinal strangulation at 6 h after operation, Chiu's score was statistically significant (P=0.001), and the serum D-LAC level was significantly increased (P=0.002). At 8 h, the intestine gradually became necrotic, and the serum D-LAC level was further increased (P=0.012), the serum I-FABP level was also significantly increased (P=0.001), and the expression of I-FABP in intestinal tissue was significantly increased (P=0.002). At 12 h, the intestine showed obvious characteristics of intestinal necrosis, Chiu's score was statistically significant (P=0.001), and the serum D-LAC and I-FABP increased to the highest level [(2019.60±16.17)μg/L vs (273.18±14.63)μg/L, P=0.001; (1210.94±5.96)μg/L vs (220.46±9.63)μg/L, P=0.001]. The expression of I-FABP in intestinal tissue was the highest [(8.20±0.60)μg/L vs (1.13±0.16)μg/L, P=0.001]. Conclusion The increased serum levels of I-FABP and D-LAC provide clinical evidence for the early diagnosis of intestinal necrosis in incarcerated hernia.
