Based on TLR-4/NF-κB signaling pathway and AQP1 expression to explore the effector mechanism of intervention of Daxianxiong decoction on early lung injury in severe acute pancreatitis
WANG Tian-lin,QU Peng-fei,GAO Wan-peng
Department of Emergency, the Second Affiliated Hospital of Tianjin University of TCM, Tianjin300150, China
Abstract:
Objective To investigate the effects and the underlying mechanism of lung injury in the early stage of severe acute pancreatitis treated with daxianxiong decoction. Methods a total of 60 rats were randomly divided into experimental, model, and control groups, with 20 rats in each group. The rats in the experimental group were intragastrically administrated with daxianxiong decoction, the control group and the model group were intragastrically administered with distilled water, and after 7 days of continuous gavage, the model group and the experimental group were treated with sodium taurocholate by pancreatobiliary injection to prepare the sap model. At each time point of 12 h, 24 h, 48 h, and 72 h after the model, five rats were sacrificed and the general condition, thoracic and abdominal water volumes, lung wet and dry specific weights, pancreatic and lung histopathological changes, lung IL-1 and TLR-4 levels, and lung NF-κB and AQP1 expression levels. Results compared with the control group, the rats in the model group were in poor general condition, and had more severe pancreatic inflammatory reaction as well as lung injury, thoracic and abdominal water volumes, lung wet and dry specific weights, and lung tissue NF-κB, the levels of IL-1 and TLR-4 were increased (P<0.05), while AQP1 expression was decreased in lung tissue. Compared with the model group, the rats in the experimental group generally had better general condition and less severe lung injury, as indicated by the lower amount of thoracic and abdominal water, lung wet and dry specific weights, and the NF-κB of lung tissue expression levels, IL-1 and TLR-4 levels were decreased (P<0.05), AQP1 expression levels were increased in lung tissue, and the degree of necrosis in pancreatic tissue at 72 h after modeling was significantly improved compared with the same period model group. Conclusion the early lung injury in SAP rats was alleviated by Daxianxiong Decoction to some extent, and the mechanism may be related to the inhibition of TLR-4/NF-κB inflammatory signaling pathways that enhance AQP1 expression are involved.
