Study on molecular mechanism of oridonin promoting apoptosis of bladder cancer
ZHAO Fan,ZHONG Zun-he,WANG Jin-rui
Department of Urology, the Second Affiliated Hospital of Hainan Medical College, Haikou 570311, China
Abstract:
Objective To investigate the antitumor effect of oridonin on bladder cancer (BC), and its molecular mechanism. Methods CCK 8 assay, Annexin V-FITC apoptosis assay, colony formation and Transwell migration assays were used to detect the effects of Oridonin on the proliferation, migration, apoptosis and colony formation of T24, respectively. Western blotting or RT-PCR was used to detect the effect of oridonin on the expression of apoptosis-related proteins, transaminase transient receptor potential channel 7 (TRPM7) and signaling molecules in T24 cells. Results After treatment of bladder tumor T24 cells with oridonin, the proliferation rate, the number of migrated cells and the colony formation rate of T24 cells were significantly decreased. The protein levels of p53 and cleaved caspase-3 in the experimental group were higher than those in the control group, and the expression levels of TRPM7 and p-AKT in the experimental group were lower than those in the control group, and their expression levels changed in a concentration-dependent manner. Conclusion Oridonin has obvious anti-proliferation and anti-migration effects on T24 cells, and the mechanism may be achieved by inhibiting of TRPM7, and inactivating AKT signaling pathway.
