miR-7-5p Affects Proliferation and Migration of Gallbladder Carcinoma Cells by Targeting FGFR4 Inhibition
LI Yun-chao，SUN Zhan-feng，SU Bin
Abstract:
Objective To investigate the effect and mechanism of miR-7-5p on the proliferation and migration of gallbladder cancer cells. Methods GBC-SD cells were selected to detect the expression of miR-7-5p and fibroblast growth factor receptor 4 (FGFR4). The miRNA regulating FGFR4 was retrieved from TargetScan database, and the binding of FGFR4 to miR-7-5p was detected by dual luciferase gene detection system. The miR-7-5p was transfected into GBC-SD cells by lentivirus to detect the expression of FGFR4, miR7-5p mRNA and FGFR4 protein, cell activity by MTT and cell invasion by Transwell. Results The expression of miR-7-5p was low and FGFR4 was high in GBC-SD cells (P<0.05). TargetScan predicted that FGFR4 specifically bound miR-7-5p and was verified in the dual luciferase gene detection system. After lentivirus transfection of miR-7-5p into GBC-SD cells, the expression levels of FGFR4 mRNA and protein decreased, the cell proliferation rate decreased and the number of transmembrane cells decreased (P<0.05). After silencing miR-7-5p, the expression levels of FGFR4 mRNA and protein increased, the cell proliferation rate increased and the number of transmembrane cells increased, with statistical significance (P<0.05). Conclusion miR-7-5p inhibits the proliferation and migration of gallbladder cancer cells by targeting FGFR4.
