Network-based Mechanistic Investigation of Fuzheng Yiliu Fomula' s Treatment of Prostate Cancer
GAN Shu,WANG Shu-sheng,CHEN Zhi-qiang
Abstract:
Objective Using network-based pharmacological methods to illustrate the potential mechanisms and molecular targets by which Fuzheng Yiliu Fomula treating prostate cancer. Methods TCMSP database were searched to obtain compounds and targets of Fuzheng Yiliu Fomula. Gencards database was searched to obtain prostate cancer-related genes. The protein interaction (PPI) of the co-targets of Fuzheng Yiliu Fomula and prostate cancer were analyzed via STRING 11.0 database. Gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were performed via DAVID 6.8 database. Cytoscape 3.8.0 was employed to construct and analyze the network among fomula, drugs, compounds, targets and pathways. Results 158 compounds and 255 compound-related targets were obtained from TCMSP. 11,719 prostate cancer-related genes were downloaded from Genecards. 233 co-targets between Fuzheng Yiliu Fomula and prostate cancer were screened out. Quercetin, kaempferol and luteolin were the main active components of Fuzheng Yiliu Fomula. PTGS2, NCOA2, PTGS1 were proposed to be primary targets for Fuzheng Yiliu Fomula in treatment of prostate cancer. Co-targets were mainly enriched in PI3K-AKT signaling pathway, regulating different biological processes including cell proliferation, apoptosis and gene transcription. Conclusion The network-based mechanistic investigation has uncovered the underlying mechanisms and potential targets by which Fuzheng Yiliu Fomula treating prostate cancer. The results are useful clues for the further experimental research.
