Based on RAS/RAF/ERK Signaling Pathway to Explore the Mechanism of YiQi ShengJi Mixture on the Formation of Microangiogenesis in the Wound Surface of a Pressure-Injured Rat Model
XIAO Si-min,XU Feng-ying,LIAO Ruo-yi
The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410208, China
Abstract:
Objective To observe the effect of Yiqi Shengji Mixture on wound repair and microangiogenesis in a rat model of pressure injury by regulating the RAS/RAF/ERK signaling pathway. Methods A rat model of stress injury and intervene was established in different ways. The wound healing and pathological damage of rats were recorded in each group, then the blood perfusion level and microvessel density (MVD) of the wound were detected in each group. Western blotting method and real-time quantitative PCR detection method was used to detect Ras and Raf-1 in the wound tissue, ERK1/2, VEGF and other proteins and mRNA expression. Results Compared with the model control group, the pressure-injured wounds of the rats in the positive drug group, the Yiqishengji group and the combined medication group all showed a significant healing trend. Among them, the combined medication group had the highest wound healing rate (P < 0.05). HE staining results also confirmed that the pathological remission of the combined medication group was the best (P < 0.05). Both Yiqi Shengji mixture and triethanolamine cream could effectively promote the blood perfusion and microangiogenesis in the wounds of rats. Among them, the combined drug group had the best effect (P < 0.05). Western blotting and RT-PCR results showed that the positive drug group, the Yiqishengji group and the combined drug group could significantly increase the Ras and Raf-
