Study on Network Pharmacological Mechanism of Simiao Pills in Treating Superficial Thrombophlebitis
GONG Rui,LIU Xiang,ZHANG Yue
Abstract:
Objective To explore the mechanism of Simiao pill in treating superficial thrombophlebitis (STP) by network pharmacology. Methods The Chinese medicine system pharmacology technology platform (TCMSP) database was used to screen the effective components of Simiao pill and predict its target. The relevant targets of STP were selected through GeneCards, OMIM, CTD and other databases. The intersection targets were obtained by intersecting the component action targets with disease targets. The TCM compound-component-targetdisease network diagram was constructed using Cytoscape 3.7.2 software, and the protein-protein interaction (PPI) network was constructed through String database. GO function and KEGG pathway enrichment analysis were performed for Simiao pill treatment of STP using the ClueGO plugin, and some compounds and targets were selected for molecular docking validation. Results 60 active ingredients, 132 potential targets of Simiao pills and 4148 targets of STP were obtained. 214 biological processes were obtained from GO Biological Process analysis, and 29 pathways were obtained from KEGG analysis. Molecular docking results showed that quercetin and β-sitosterol had good docking activity with AR, ESR1 and F2. Conclusion Simiao pills may treat STP by acting on androgen receptor (AR), estrogen receptor 1 (ESR1), thrombin II (F2) and other targets as well as the role of interleukin-17 (IL-17) signaling pathway, tumor necrosis factor (TNF) signaling pathway, relaxin (RLX) signaling pathway and C-type lectin receptor (CLR) signaling pathway and protecting vascular endothelial cells through the active ingredients such as mistletoe bombesin (MOL000098) and β-sitosterol (MOL000358), reflecting the synergistic mechanism of Simiao pills in the treatment of STP with multiple molecules, multiple targets and multiple pathways, providing a theoretical basis for the subsequent in-depth discussion of the molecular mechanism of Simiao pills in the treatment of STP.
