Study on Screening Differential Protein of Wound in Diabetic Mice with High Throughput Chip
LIU Peng,XU Yang,LIU Guo-tao
Abstract:
Objective To investigate the possible mechanism of diabetic wound recovery by screening the differential protein between diabetic mice and normal mice with high-throughput protein microarray.Methods There were 20 healthy BALB/c male mice, 10 of them were randomly selected as the normal group and the remaining 10 were randomly selected as the diabetic group. The diabetic model was prepared by STZ injection combined with high fat and high sugar, and the wound model was prepared by full-thickness skin resection. The samples were collected on the third day after the successful replication of the wound model.GSMCAA-4000 microarray was used to quantitatively detect the content of 200 protein factors in the wound tissues of two groups of mice. Results The diabetic mouse model was successfully prepared by high fat and high sugar feeding combined with intraperitoneal injection of STZ. The body weight of the diabetic wound group decreased significantly (P < 0.05), and the blood glucose of the diabetic wound group was higher than that of the normal group (P < 0.01).GSM-CAA-4000 microarray was used to quantitatively detect 200 proteins in the wound surface of mice of the two groups, and a total of 29 different proteins were screened out.Compared with the normal group, the protein factors that increased in the diabetic group included ACE,GAS 1, IL-33, P-cadherin, E-selectin, 6Ckine, IL-1ra, Periostin, VEGF-D, Dtk, MCSF, SDF-1a, VEGF,TARC, Pro-MMP-9, gp130, VCAM-1, TNF RI(P < 0.05). The reduced protein factors included MBL-2, CD36, SHH-N, Renin 1, Fetuin A, Limitin, IL-10, VEGF-B, Persephin and TRANCE (P < 0.05). Conclusion A total of 29 differential proteins were screened by protein chip technology, mainly involving growth factors, chemokines, adhesion molecules, tumor necrosis factor and receptor families, suggesting abnormal cell proliferation, chemotaxis, migration adhesion and matrix degradation in diabetic wounds.
