Effect of MMP-9 Expression Inhibition by Norcantharidin Interference on Migratory and Invasive Ability of PC-3 cells
LUO Xiang-ning,WANG Wen-juan,LI Kai
Abstract:
Objective To study the effect of norcantharidin on the invasion of PC-3 cells. Methods When norcantharidin was applied to PC-3 cells, Western blot and real-time fluorescence quantitative PCR were used to detect the expression level of MMP-9.The scratch test and invasion test were used to detect the invasiveness of PC-3 cells, and transmission electron microscopy was used to observe the morphological changes of invasive pseudopods of PC-3 cells. Results The mRNA expression of MMP-9 in PC-3 cells of norcantharidin intervention group was significantly lower than that in PC-3 cells of blank control group. The expression of MMP-9 in PC-3 cells of the norcantharidin intervention group was significantly lower than that of the blank control group (P<0.05).The scratch test results showed that the scratch healing degree of PC-3 cells in the norcantharidin intervention group was significantly lower than that of the blank control group (P<0.05). The invasion ability of PC-3 cells in the norcantharidin intervention group was significantly lower than that of the blank control group (P<0.05). The transmission electron microscope observation showed thatthe invasion of pseudopodia by PC-3 cells in the norcantharidin intervention group was significantly less than that in the blank control group. Conclusion After norcantharidin acts on PC-3 cells, the invasion of PC-3 cells decreases, which may be related to the decrease of the expression level of MMP-9.
