Effects of Dexmedetomidine and Sufentanil Combined Intrathecally Injection on GABAA Activation Current in DRG Neurons of CCI Model Rats
SHU Luo-wa,WANG Gu-yan
Department of Anesthesiology, Capital Medical University Affiliated Beijing Tongren Hospital, Beijing 100730, China
Abstract:
Objective To investigate the analgesic effect of combined dexmedetomidine and sufentanil intrathecal injection on CCI model rats. Methods Intrathecal catheters were successfully inplanted in 50 male SD rats.Fifty male SD rats with intrathecal catheters were randomly divided into 5 groups(n=10 per group): sham group(Sham), model control group(CCI group), dexmedetomidine group(Dex group), sufentanil group(Suf group) and combined treatment group(DS group). Intrathecal injection was given 1 to 14 days after operation. The rats in Dex group was injected with dexmedetomidine 2μg/kg. The rats in Suf group were injected in with sufentanil 1μg/d, The rats in DS group were injected in with dexmedetomidine1μg/kgandSufentanil 0.5μg. The total capacity of each group of drugs was diluted into 20μL. The rats in sham group and CCI group were intrathecally injected with an equal volume of 0.9% saline daily. The paw thermal withdrawal latency(TWL)and mechanical withdrawal threshold (MWT) were measured 30 min after intrathecal injection preoperative 1 day and 1, 3, 7, 14 days after CCI the 7th day after the operation. The TWL and MWT were measured, and the dorsal root ganglion cells of L4 to 6 rats were extracted andisolated (n = 9 per group). Whole cellpatch clamp technique was used to detect GABAactivation current in rat dorsal root cells. Results Compared with sham group, the TWL and MWT in CCI group, Dex group, Suf group and DS group were significantly decreased on the3rd day, the 7thday and the 14thday after surgery (P<0.05).Compared with CCI group, the TWL and MWT in Dex group, Suf group, DS group, and DS group were signi?cantly increased on the 3rdday, the 7th day and the 14th day after CCI (P<0.05). Compared with DS group, the TWL and the MWT in Dexgroup and Suf group were signi?cantly decreased on the 3rd day, the 7thday and 14th day after CCI (P<0.05). Compared with CCI model group, IGABA in Dex group, Suf group and DS groups were signi?cantly increased at 7 days after operation (P<0.05). Compared with DS group, IGABA in Dex group and Suf group were signi?cantly decreased (P<0.05). Conclusion Combined intrathecal application of dexmedetomidine and sufentanil in the treatment
