PFKFB4 Promotes the Proliferation and Migration of Ovarian Cancer Cells Through Regulating Glycolysis
GAO Rui-fang,XUN Jing,LI Ning-xin
Abstract:
Objective To investigate the effects and mechanism of 6-phosphofructo-2-kinase/fructose-2,6biphosphatase-4 (PFKFB4) on the proliferation and migration of ovarian cancer cells. Methods PFKFB4 knockdown and overexpression A2780 stable cell lines were established using lentivirus. The knockdown and overexpression efficacy of PFKFB4 was detected by qPCR and Western blotting. The proliferation, migration and glycolysis level of A2780 cells were detected by CCK-8 assay, clone formation assay, scratch healing assay, Transwell assay, flow cytometry and kits respectively. Results A2780 stable cell lines with PFKFB4 knockdown and overexpression were successfully established. Compared with the control group, PFKFB4 mRNA and protein levels, proliferation and migration ability, glucose consumption and lactic acid secretion levels of A2780 cells were significantly decreased after PFKFB4 knockdown (P<0.05), and ROS level was significantly increased (P<0.05). After overexpression of PFKFB4, the levels of PFKFB4 mRNA and protein, proliferation and migration ability, glucose consumption and lactic acid secretion of A2780 cells were significantly increased (P<0.05), and the ROS level was significantly decreased (P<0.05). Conclusion PFKFB4 promotes the proliferation and migration of ovarian cancer cells through glycolysis.
