Effect and Mechanism of Rhein on Proliferation and Migration of Human Pancreatic Cancer Cells
HU Li-juan,WANG Feng
The Institute of Integrative Medicine for Acute Abdominal Diseases, Tianjin Nankai Hospital, Tianjin 300100, China
Abstract:
Objective To study the effect and mechanism of rhein on proliferation and migration of human pancreatic cancer cells. Methods Human pancreatic cancer MiaPaCa-2 cells were treated with different concentrations of rhein and the proliferation of MiaPaCa-2 cells was assessed by CCK8 method. MiaPaCa-2 cells were cultured in normoxic and hypoxic conditions and cells migration was examined by transwell. Hypoxia inducible factor-1α (HIF-1α), E-cadherin and Zinc Finger Transcription Factor (Snail) were determined by western blot assay. Results The results of CCK8 assay showed that rhein inhibited the proliferation of pancreatic cancer MiaPaCa-2 cells in a dose- and time-dependent manner (P<0.05). Rhein also inhibited the migration of MiaPaCa-2 cells in both normoxic and hypoxia conditions (P<0.05). Compared with the control group, rhein inhibited the expression of HIF-1α and Snail in adose-dependent manner and rhein could increase the expression of E-cadherin (P<0.05). Conclusion Rhein can inhibit the proliferation and migration of human pancreatic cancer cells, which may be related to the inhibition of HIF-1α expression in MiaPaCa-2 cells.
