Study on Metabolic Pathway of Sijunzi Decoction after Intestinal Obstruction Relief Based on Network Pharmacology
ZHENG Zhang-qiang,WANG Tong-tong,LI Lin
Abstract:
Objective Mechanical intestinal obstruction is one of the common diseases in abdominal surgery.Most of them need surgical treatment. After operation, there are obvious intestinal dysfunction, systemic immune imbalance and nutritional disorder. It is found that Sijunzi decoction has a good function in the preparation of the state when the intestinal obstruction is relieved, but the mechanism is not clear. The study of metabolomics and network pharmacology reveal the mechanism after the operation of mechanical intestinal obstruction (MIO).Methods We divided the rats into different groups based on whether to feedSijunzi decoction or not. We used ultra-high performance liquid chromatography and UPLC-Q-TOF/MS technology to study the metabonomics of the rat plasma samples, compared and analyzed the levels of metabolites in the plasma samples of different groups and used the network pharmacology method to find the metabolites which related to metabolic pathways.Results The results showed that the metabolism of rats in Sijunzi decoction group changed significantly. As the time went on, the metabolism of rats in Sijunzi decoction group tended to be faster than that in the control group. Finally, it was concluded that the intervention of Sijunzi decoction on rats was mainly related to the metabolism of arginine and proline, glycerophospholipid and sphingolipid. Conclusion Sijunzi decoction has a good intervention effect on intestinal dysfunction, systemic immune imbalance and nutritional disorders after intestinal obstruction relief, which mainly through the influence of arginine metabolism pathway and phospholipid metabolism pathway.
