Effect of Protein Kinase C Inhibitor on TLRs and NF-κB Induced by High Glucose in Fibroblasts
SHANG Ting-ting,YAN Hui
Department of Stomatology, Tianjin Hospital of Integrated Traditional Chinese and Western Medicine, Tianjin300000, China
Abstract:
Objective To explore the effect of PKC siRNA on TLRs and NF-κB of TLR signaling pathway induced by high glucose in fibroblasts. Methods The expressions of TLR2 and TLR4 were detected by realtime PCR under the stimulation of high glucose. The PKC-α siRNA, PKC-β siRNA and PKC-δ siRNA were applied to block PKC subunit. The expressions of TLRs, NF-κB p50 and NF-κB p65 were detected by realtime PCR method after the stimulation of high glucose in comparison to the low glucose we cultured. Results The mRNA level of TLR2 in HGFs under the stimulation of high glucose significantly increased (P <0.05), while the mRNA level of TLR4 had no obvious change (P >0.05). PKC-α siRNA and PKC-δ siRNA down-regulated mRNA levels of TLR2 and NF-κB p65 (P <0.05) induced by high glucose, however, the PKC-β siRNA showed no effect on the mRNA levels of TLR2 and NF-κB p65 (P >0.05). There was no change in the level of NF-κB p50 (P >0.05) under the stimulation of high glucose compared to the low glucose cultured. Besides, PKC-αsiRNA, PKC-β siRNA and PKC-δ siRNA did not change the level of NF-κB p50. Conclusion It indicates that PKC-α and PKC-δ is the up-stream of TLR2 and NF-κB p65.
