The Protective Effect of Dachengqi Decoction on Intestinal Mucosal Barrier of Rats after Intestinal Obstructi on Relief
MA Jun-hong,LI Dong-hua,LIU Jin-jin
Department of Gastrointestinal Surgery 1,Tianjin Nankai Hospital, Tianjin300100, China
Abstract:
Objective To study the protective effect of Dachengqi decoction on intestinal mucosal barrier of rats after intestinal obstruction relief. Methods We have established recoverable complete intestinal obstruction model through external compression using the non-damaging intestinal forceps, 30 Wistar rats were randomly divided into 3 groups. 6 rats in control group, 12 in model groupand 12 in Traditional Chinese medicine group. For Chinese medicine group, the rats were given objects enema with Dachangqi decoction (1mL/100g)twice per day for 96 hours, after intestinal obstruction relief. For Model Group, the rats were given objects the same does of enema with Saline (1 mL/100g) twice per day for 96 hours. Done blood test to measure the level of Serum D-lactic acid 48 hours and 96 hours after intestinal obstruction relief. For each group, we used RT-PCR to test the expression level of the tight junction protein on epithelia of small intestineClaudin-1mRNA. Results After 48 hours and 96 hours of intestinal obstruction relief, there were no statistical differences between the level of Serum D-lactic acid of objects in model group and control group (P >0.05). There was significant decline of level of Serum D-lactic acid of objects in Chinese medicine group after 48 hours and 96 hours of intestinal obstruction relief, which showed statistical difference(P <0.05). This result illustrated a notable downtrend of level of Serum D-lactic acid after giving enema with Dachengqi decoction. After intestinal obstruction relief,there was a decreasing trend of level of Claudin-1mRNA on intestinal mucosa of objects in control group,showing a significant decline of its expression level. In contrast, the level of Claudin-1mRNA on intestinal mucosa of objects in Chinese medicine group changes obviously after 48 hours and 96 hours, compared to that of objects in control group, showing a statistical difference(P <0.05 ). Conclusion Dachengqi decoction can improve the intestinal mechanical barrier function of Wistar rats after intestinal obstruction relief.
