Effects of Glaucocalyxin B on Cell Proliferation, Migration and Invasion of Triple-negative Breast Cancer Cells
WU Yuan-zhao,JIN Zao
Department of Oncological Surgery, Wenzhou People' s Hospital, Wenzhou325000, China
Abstract:
Objective To investigate the effects of Glaucocalyxin B (GLB) on the proliferation, migration and invasion of MDA-MB-231 cells in triple negative breast cancer. Methods MDA-MB-231 cells were cultured in vitro and then MTT assay was used to detect cell proliferation. The cell migration ability was investigated by scratch test. Transwell assay was used to test the ability of cell invasion. Western blotting was used to analyze the expression levels of p38MAPK, p-p38MAPK, FOXO3α and related markers of epithelial-mesenchymal transition (EMT). Results With the increase of GLB concentration (2, 4 and 8 μmol/L) , cell proliferation rates [(83.2±6.90)%, (70.72±6.53)% and (45.43±7.51)%] were signi?cantly decreased compared with control group [(100.00±7.84)%, P < 0.05, P < 0.01]. The numbers of invasive cells [(300.54±24.91), (255.44±23.59) and (208.66±36.18)] were signi?cantly decreased compared with those of the control group [(368.44±28.32), P < 0.05, P < 0.01]. Cell migration distances [(487.11±53.00)μm, (394.93±61.91)μm and (312.88±35.42)μm] were signi?cantly decreased compared with those of the control group [(559.37±75.77)μm, P < 0.05, P < 0.01]. p-p38MAPK expression [(1.38±0.11), (1.69±0.13) and (2.23±0.19)] was signi?cantly increased compared with that of the control group [(1.00±0.09), P < 0.05, P < 0.01]. FOXO3 α expression [(1.40 ± 0.16),(1.97±0.31) and (2.44±0.26)] were signi?cantly comincreased compared with those of the control group [(1.00±0.18), P < 0.05, P < 0.01]. E-cadherin expression [(1.15±0.11), (1.77±0.22) and (1.86±0.15)] was signi?cantly increased compared with that of the control group [(1.00±0.11), P < 0.05, P < 0.01]. Vimentin expression [(0.86±0.04), (0.49±0.05) and (0.54±0.04)] was signi?cantly decreased compared with that of the control group [(1.00±0.04), P < 0.05, P < 0.01]. N-cadherin expression [(0.66±0.07), (0.58±0.08) and (0.42±0.04)] was signi?cantly decreased compared with that of the control group [(1.00±0.12), P < 0.05, P < 0.01]. Conclusion GLB can effectively interfere with the EMT process of tumor cells by mediating p38MAPK/FOXO3a signaling pathway and it finally plays an important role in inhibiting the proliferation, migration and invasion of triple-negative breast cancer cells.
