Effect and Mechanism of Senkyunolide A on Renal Interstitial Fibrosis in Unilateral Ureteral Obstruction
Rats ZHENG Lei,CHEN Xiang,YIN Bo-wei
Abstract:
Objective To study the renal protective effect of Senkyunolide A(SenA) in rats with unilateral ureteral obstruction(UUO). Methods 50 male SD rats were randomly divided into the sham operation group,the model group, the Sen A low dose (20 mg/kg) group, the SenA high dose (40 mg/kg) group and the positive control group (Irbesartan, 20 mg/kg). The unilateral ureter model was prepared by UUO and the rats were killed on the 21th day after operation. According to the kit instruction, the serum content of Scr and BUN were detected.HE staining was used observing the renal pathological changes.Immunohistochemical method was used detecting kidney tissues of α-SMA protein expression. Western blotting analysis was used measuring Wnt4, β-catenin,p-GSK-3β and E-cadherin expression level. Results Whencompared with the model group, theserum contents of Scr and BUN in each treatment group were significantly reduced (P <0.05), and the kidney damage was improved to different degrees. At the same time, the SenAcouldobviously reduce the α-SMA in rat kidney(P <0.05), down-regulated the Wnt4, β-catenin and p-GSK-3βexpression levels (P <0.05), accompanied with E-cadherin protein expression significantly up-regulated (P <0.01). Conclusion Senkyunolide A can downregulate Wnt4/β-catenin signaling pathway, inhibit extracellular matrix deposition in renal tissue, improve renal function, and ultimately reverse the process of renal interstitial fibrosis in UUO rats.
