Protective Effect and Mechanism of Hongteng Decoction on Mice with DSS-induced Ulcerative Colitis
LIULi-hua,CAO Ze-wei
Tianjin University of Traditional Chinese Medicine, Tianjin (301617), China
Abstract:
Objective To observe the anti-inflammatory and anti-oxidative effects of Hongteng Decoction mice with ulcerative colitis induced by Dextran sulphate sodium (DSS), and to clarify the protective mechanism of Hongteng Decoction on ulcerative colitis. Methods Thirty healthy and clean BALB/c mice were randomly divided into three groups (n =10): normal control group (group C), DSS-induced ulcerative colitis model group(group M), and model + Hongteng Decoction group (group H). Group C was free to drink water. Group M and Group H were freely drinking 4% DSS to establish ulcerative colitis model. Group H was administered from the first day of modeling. After 10 days of continuous administration, arterial blood was collected for TNF-a and the concentration of IL-6, colon histopathological score, colorimetric determination of MDA amount and SOD and GSH-PX activity, Western blot detection of ERK1/2, P-ERK1/2, JNK, P-JNK and GADPH protein Expression. Results Compared with group M, colonic pathology scores and serum inflammatory factors(TNF-α and IL-6) levels were significantly lower in group H (P <0.05); MDA level in colon tissues was decreased (P <0.05), while SOD and the activity of GSH-PX increased (P <0.05), and the expression levels of P-ERK1/2 and P-JNK were also significantly down-regulated (P <0.05). Conclusion Hongteng Decoction has protective effect on DSS-induced ulcerative colitis in mice. It can up-regulate antioxidant genes, reduce free radical production, re-establish the redox balance. In addition，Hongteng Decoction also inhibits the activation of JNK and ERK1/2 signaling pathways, reduces the production of inflammatory factors, and eventually inhibits
