Effects of QianlieXiaozheng Decoction on Apoptosis and Cell Cycle of DU-145 Cells
QI Lei-lei,ZHANG Li-juan,ZHOU Bing-ying
Department of Urology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China
Abstract:
Objective To observe the effect of QianlieXiaozheng Decoction (QXD) on the cell cycle and apoptosis of DU-145 cells. Methods The cell cycle and apoptosis of DU-145 cells under different concentration of QXDwere researchedby flowcytometry. Results The early stage of apoptosisandlate stageof apoptosis in medium dose group both had statistical differences compared with control group(P<0.05). The early stage of apoptosisandlate stageof apoptosis in high dose group both had statisticallysignificantdiff erences compared with control dose group(P <0.01).The result of cell cycle showed that the ratio of cells in Speriod in control group and medium dose group was (31.69±1.29)% and(37.96±2.24)% respectively,with statistically significant difference(P <0.05). The ratio of cells in S period in control group and high dose group was (31.69±1.29)%and(43.77±2.48)% respectively, with statistically significantdifference(P<0.01). Conclusion QXD could induce the apoptosis of early stage and late stage of DU-145 cells. The induction augmented which was followed by the concentration increasing. The results predicted that the QXD could stop the cell cycle of DU-145 cells and turn S period into G2 period.
