Expression of NF-κB and P53 in Bladder Cancer and Relationship with Clinicopathological Features
GAO Chao,GAO Yan,DU Zhang-min
Department of Oncology, Pidu District Peopoe' s Hospital of Chengdu City, Chengdu 611730, China
Abstract:
Objective To investigate the expression of NF-κB and P53 in bladder urothelial carcinoma and the relationship with patients' clinicopathological features and prognosis. Methods Forty-eight patients with bladder urothelial carcinoma treated with surgery and 16 non-bladder cancer cases were included in this study from February 2014 to January 2017 in Pidu District People' s Hospital of Chengdu. The expression levels of NF-kB and P53 protein in bladder urothelial carcinoma, adjacent normal bladder tissues of bladder urothelial carcinoma patients and normal urinary tract mucosa of non-bladder cancer patients were examined by immunohistochemical SP assay. According to NF-kB and P53 protein expression, the bladder urothelial carcinoma patients were divided into positive and negative expression group. The relationship between NF-kB, P53 protein expression and patients’ clinical characteristics (including age, sex, pathological grade, clinical stage, lymph node metastasis and recurrence after operation) was evaluated. Results The positive expression rates of NF-kB protein were 52.5% (30/48), 16.7% (8/48) and 6.3% (1/16) for bladder urothelial carcinoma tissue, corresponding tissue and normal urinary tract mucosa respectively. For P53, the positive rates were 72.9% (35/48), 45.8% (22/48) and 12.5% (2/16) for the above three types of tissues. The positive expression rates of NF-kB and P53 had statistical difference among the three types of tissues (P<0.05). The positive expression of NF-kB in high grade bladder urothelial carcinoma (G2/G3) was significantly higher than that of low grade one (G1) (P<0.05); The positive expression of P53 was correlated with the stage of bladder urothelial carcinoma, and the positive rate of T2–T3 was signi.cantly higher than that of Tis-T1 (P<0.05); Disease free survival (DFS) was 8.9 months and 30.1 months respectively for NF-κB positive and negative group, which indicated the recurrence risk of positive group was signi.cantly higher than that of negative group (P<0.05); DFS of P53 positive and negative patients was 9.2 months and 28.6 months, respectively. The recurrence risk of positive group was also significantly higher than that of negative group (P<0.05). Conclusion Compared with normal bladder mucosa, the expression of NF-kb and P53 protein in patients with bladder urothelial carcinoma is signi.cantly increased, which is related to the grade and stage of bladder cancer, and can be used as a molecular marker for predicting the recurrence of bladder urothelial carcinoma after operation.
