Effects of Astragalus Polysaccharides on Proliferation and Expression of VEGF in Human Umbilical Vein Endothelial Cells
HE Li-hong,ZHENG Xuan,MO Jia-hang
Xiaoshan Hospital of Traditional Chinese Medicine of Hangzhou City, Hangzhou (311201), China
Abstract:
Objective To explore the effect of Astragalus polysaccharide (APS) on the cell cycle of human umbilical vein endothelial cells (HUVEC) and the expression of vascular endothelial growth factor (VEGF). Methods HUVEC were cultured with different concentration of APS, and the cell cycle and apoptosis were detected by flow cytometry. Immunofluorescence staining of VEGF in cells was detected by fluorescence microscope. Results MTT assay showed that APS promoted the cell cycle of HUVEC from G0/G1 phase to G2/M phase and S phase within a certain concentration range. Flow cytometry apoptosis assay showed that APS did not increase the apoptosis rate of HUVEC. Cellular immuno?uorescence staining showed that APS promoted VEGF expression. Conclusion APS could promote division and proliferation of HUVEC at a certain concentration range (0.1–100 μg/mL). In addition, APS can also up-regulate the expression level of VEGF in HUVEC, providing an effective angiogenic factor for further vascularization and a new idea for the treatment of ischemic diseases.
