Effects of Bufei Decoction on 5-ht and SP in Colon of Rats with Slow Transit Constipation
CHEN Xian-tao,DU Wei-liang
Guang`an People' s Hospital, Guang`an (638000), China
Abstract:
Objective To observe the effect of Bufei Decoction on the expression of 5-hydroxy tryptamine (5-HT) and substance P (SP) in colon of STC model rats, and to provide animal experimental basis for treating STC from the lung based on the theory of "the lung and large intestine". Methods SD rats were randomly divided into blank control group (group A), model control group (group B) and Bufei Decoction treatment group (group C), with 10 rats in each group. The STC rat model was established by DC gavage in groups B andC. The ra ts in group C were treated with Bufei Decoction. After that, activated carbon propulsion experiment was used to detect various intestinal transmission functions of rats. Immunohistochemistry was used to detect the expression of 5 -HT and SP in colon tissues of rats in each group. Results In group C, the first black defecation was excreted for (318.2±25.1) min, the number of feces at 24 h was 12.7±2.6, and the wet weight of feces at 24 h was (34.9±4.4) g. The mean optical density of 5-HT (0.159±0.011) and SP (0.156±0.006) in the colon tissues of rats treated with Bufei Decoction were both higher than that of the model control group (P<0.05). Conclusion Bufei Decoction can regulate the expression levels of 5-HT and SP in the colon of STC model rats.
