Expression of KIF18A in Colorectal Carcinoma and its Relationship with Clinicopathological Features
LI Bin,FENG Lian-zhong,WANG Chun-hua,BAO Tie,ZHENG Li,DONG Lai-rong
The Second Affiliated Hospital of Jiaxing University, Jiaxing (314000), China
Abstract:
Objective To explore the expression of KIF18A in colorectal carcinoma tissues and its relationship with various clinicopathological parameters and prognosis of the patients. Methods The fresh paired colorectal cancer tissues and adjacent normal tissues (> 5 cm from the edge of tumor) from 35 colorectal cancer patients undergoing operation from Jan. 2016 to Jun. 2016 were collected. The relative expression of KIF18A at mRNA level was detected by real-time fluorescence quantitative Polymerase Chain Reaction. Clinicopathological and follow-up data of 92 patients with colorectal carcinoma undergoing operation from Jan. 2012 to Jul. 2012 were retrospectively analyzed. The expression of KIF18A protein in samples of 92 resected colorectal cancer tissues and 20 normal colorectal tissues was detected by immunohistochemisty. The expression rates of KIF18A were compared among different clinicopathological features. Moreover, the association between KIF18A expression and prognosis was analyzed. Results The relative expression quantity of KIF18A at mRNA level in colorectal cancer tissues (4.056±0.4024) was signi?cantly higher than that in adjacent normal tissues (1.253 ±0.1438) with signi?cant difference (P < 0. 01). The expression of KIF 18 A protein located mainly in cytoplasm. Positive rate of KIF 18 A expression in colorectal cancer tissues was 80% (74/92), which was obviously higher than that in normal colorectal tissues ( 15 %,3 / 20 ) with significant difference (χ 2 = 32 . 741 , P < 0 . 01 ). The analysis on clinicopathological parameters suggested that the expression level of KIF18A in cancer tissues was associated with invasion depth (χ2=15.031, P<0.01), lymph node metastasis (χ2= 6.064, P<0.05) and pTNM staging (χ2=6.546, P<0.05). The overall 3-year survival of colorectal cancer patients with high levels of KIF18A expression was lower than that of patients with low levels of KIF18A expression (54.1% vs 100%, χ2=15.326, P<0.01). Univariate Cox regression analysis showed that tumor size (P<0.05), degree of differentiation (P<0.01), depth of tumor invasion (P<0.01), lymph node metastasis (P < 0 . 01), pTNM staging (P < 0 . 01) and KIF18A expression level (P < 0 . 01) were associated with the prognosis of colorectal cancer patients. Multivariate Cox regression analysis indicated that the level of KIF18A expression was independent factor impacting the survival of colorectal cancer patients (HR = 11. 419, 95% CI:1.504~86.684, P<0.05). Conclusion The high expression of KIF18A in colorectal cancer suggests that KIF18A may be associated with poor prognosis of patients with colorectal cancer and may be a potential marker for diagnosis, treatment and prognosis of colorectal cancer.
