Effect of Aurora Kinase A on Invasion and Migration of Gastric Cancer Cells through Wnt Signaling Pathway
WEI Xiao-dong,LIU Xi,TANG Yan-ping
Department of Gastroenterology, Tianjin Nankai Hospital, Tianjin (300100), China
Abstract:
Objective To investigate the effect of Aurora Kinase A (AURKA) on invasion and migration of gastric cancer cells through Wnt signaling pathway. Methods Gastric cancer cells SGC-7901 were treated by AURKA inhibitor, MLN8237 (20μmol/L). The expression of AURKA, β-catenin, N-cadherin, E-cadherin and Twist was detected by real-time quantitative PCR (qRT-PCR) and Western blotting.Cell invasion abilities and proliferation were detected by Transwell and clone formation assay. Results MLN8237 decreased the expression of AURKA ( 0 . 36± 0.09, P <0.01), β-catenin ( 0 . 41± 0.07, P < 0 . 01), N-cadherin ( 0 . 26± 0.08, P < 0 . 01 ) and Twist ( 0 . 33 ± 0.12, P < 0 . 01 ). However, the treatment of MLN 8237 increased the level of E-cadherin (4 .05± 0.96, P < 0 .05). And the treatment of MLN8238 induced the G 2 /M arrest (P < 0 . 05). The Transwell assay showed that the number of cells passing through basement membrane in the experimental group ( 28 . 33 ± 3 . 82 ) was significantly lower than that in the control group ( 83 . 67 ± 4.28, P < 0 . 05 ). The plate cloning experiment showed that the number of clones formed in the experimental group (104.67±5.73) was significantly lower than that in the control group ( 417 . 00 ± 7.25, P <0.05). Conclusion AURKA could inhibit the invasion and proliferation of glioma cells by down-egulating Wntsignaling pathway activity. AURKA can be used as a potential target to improve the sensitivity of gastric cancer to chemotherapy.
