Effect of Xiangxin Prescription on Inflammatory Medium and MCP-1 in Mice with Bone Metastatic Cancer Pain
HE Pei-shan, FENG Xing-zhong, JIANG Min, et al.
Department of Traditional Chinese Medicine,Beijing Shijitan Hospital, Capital Medical University, Beijing (100038), China
Abstract:
To explore the mechanism of Xiangxin Prescription on relieving bone metastatic cancer pain using in vivo fluorescence imaging model of lung cancer with bone metastasis. Methods Lung cancer bone metastatic pain model was built by injecting human lung adenocarcinoma A549 cells expressing green fluorescent proteins into the femurs of 30 nude mice. After successful modeling, the mice were randomly divided into external application of Chinese herb group, Votalin group and model group, n=10. Sham operation group was also established with other 10 mice. The external application of Chinese herb group was externally given Xiangxin Prescription, 0.5 g/(kg·d); the Votalin group was locally applied with Votalin, with the dose of 0.1 g/(kg·d); the model group and Sham operation group were embrocated with normal saline (0.5 mL). The external application in each group was kept for at least 6 h, once daily. For each group, monocyte chemotactic protein-1 (MCP-1), interleukin-lβ (IL-lβ), tumour necrosis factor α (TNFα) and interleukin-6 (IL-6) were observed after the treatment. Results The level of MCP-1 in model group [(1.88±0.18) mg/L]
was higher than that in Sham operation group [(1.21±0.19) mg/L] (P<0.05). The level of MCP-1 in Chinese herb group [(1.51±0.09) mg/L] was less than that in model group (P<0.05). The levels of IL-1β in Votalin group [(99.11±10.52) ng/L] and model group [(104.46±11.07) ng/L] were higher than that in Sham operation group [(75.69±16.57) ng/L] (P<0.05). The level of IL-1β in Chinese herb group [(85.97±11.88) ng/L] was less than those in Votalin group and model group (P<0.05). The level of TNFα in Chinese herb group [(447.91±44.67) ng/L] was less than those in Votalin group [(504.14±44.30) ng/L] and model group [(534.84±48.89) ng/L] (P<0.05). The difference of IL-6 level in each group was not significant (P>0.05). Conclusion Xiangxin Prescription could inhibit the expression of MCP-1 and reduce the levels of IL-1β and TNFα, which could help to relieve the bone metastatic cancer pain.
